Besides direct viral neutralization, antibodies can also lead to effector cell
activation and clearance of the viral antigen-expressing cells through
antibody-dependent cellular cytotoxicity (ADCC) or phagocytosis (ADCP)29.
除了直接中和病毒外,抗體還可以通過抗體依賴性細胞毒性(ADCC)或吞噬作用(ADCP)
導致效應細胞活化和表達病毒抗原的細胞清除。
ADCC/ADCP has been shown to be highly relevant for protecting against and
clearing several different viruses, including HIV, influenza virus, and
EBOV30,31,32,33,34.
ADCC / ADCP已被證明與防止和清除幾種不同的病毒(包括HIV,流感病毒和EBOV)高度相關。
Fig. 7
Lassa virus (LASV)
拉薩病毒(LASV)
LASV glycoprotein (GPC)
LASV糖蛋白(GPC)
antibody-dependent cellular cytotoxicity (ADCC)
抗體依賴性細胞毒性(ADCC)
To determine which antibody isotype is important for ADCC-mediated killing of 3T3-LASV,we isolated IgG from the sera and conducted the assay with 40 µg/ml of either purified IgG or IgG-depleted sera (Fig. 7b and Supplementary Fig. 3). Again, killing of 3T3-LASV was significantly higher in the presence of LASV-specific purified IgG than in the control;in contrast, target cell cytotoxicity was reduced to background levels when IgG-depleted sera were used. Together these findings indicate that ADCC is mediated by the LASV GPC-specific IgG.
為了確定哪種抗體同種型對於ADCC介導的3T3-LASV殺傷很重要,我們從血清中分離了IgG,
並用40μg/ ml純化的IgG或IgG耗盡的血清進行了測定(圖7b和補充圖3)。 )。
同樣,在存在LASV特異性純化的IgG的情況下,對3T3-LASV的殺滅作用顯著高於對照。
相反,當使用IgG耗盡的血清時,靶細胞的細胞毒性降低至背景水平。
這些發現共同表明ADCC由LASV GPC特異性IgG介導。
所以拉薩病毒也可藉由ADCC機制消滅。